bbsi digested pxr003 vector (Addgene inc)
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bbsi digested pxr003 vector
Bbsi Digested Pxr003 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 71 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pxr003+casrx+grna+cloning+backbone/pXR003%3A+CasRx+gRNA+cloning+backbone+(Plasmid+%23109053)/pm41928225-94-9-12
Average 95 stars, based on 71 article reviews
Bbsi Digested Pxr003 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 71 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pxr003+casrx+grna+cloning+backbone/pXR003%3A+CasRx+gRNA+cloning+backbone+(Plasmid+%23109053)/pm41928225-94-9-12
Average 95 stars, based on 71 article reviews
bbsi digested pxr003 vector - by Bioz Stars,
2026-09
95/100 stars
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Plasmid Preparation:Article Title: Development of CRISPR/Cas13-based analytical tools to study RNA-Protein Interactions Article Snippet: For crRNA production or guide RNA construction, annealed oligos were inserted into crRNA backbone vector or lentiGuide-Puro vector . crRNA sequences were designed with Cas13design ( https://cas13design.nygenome.org/ ). sgRNA sequences were designed with CRISPick ( https://portals.broadinstitute.org/gppx/crispick/public ). .. The sequences of the crRNAs and sgRNAs used in this study are described in the Appendix Table S1. pXR002: EF1a-dCasRx-2A-EGFP (Addgene plasmid # 109050; http://n2t.net/addgene:109050 ; RRID:Addgene_109050) and Article Title: Adeno-associated viral vector resource for the RNA-targeting Cas13d: A comparison of high-fidelity variants, DjCas13d and hfCas13d Article Snippet: The DNA sequence of RfxCas13d (CasRx) was derived from pXR001: EF1a-CasRx-2A-EGFP, Addgene plasmid no. 109049. .. CasRx-compatible gRNA expression cassettes used in this study were derived from Article Title: Adeno-Associated Viral Vector Resource for the RNA-Targeting Cas13d: A Comparison of High-Fidelity Variants, DjCas13d and hfCas13d Article Snippet: The DNA sequence of 399 RfxCas13d (CasRx) was derived from pXR001: EF1a-CasRx-2A-EGFP, Addgene plasmid #109049. .. CasRx-compatible 400 gRNA expression cassettes used in this study were derived from Article Title: U2AF1 mutations rescue deleterious exon skipping induced by KRAS mutations Article Snippet: Band intensities were quantified using the Analyze Gels feature in Fiji (ImageJ) and used to calculate the percent spliced in for KRAS exon 2 or exon 3. .. pXR002: EF1a-dCasRx-2A-EGFP and Cloning:Article Title: Development of CRISPR/Cas13-based analytical tools to study RNA-Protein Interactions Article Snippet: For crRNA production or guide RNA construction, annealed oligos were inserted into crRNA backbone vector or lentiGuide-Puro vector . crRNA sequences were designed with Cas13design ( https://cas13design.nygenome.org/ ). sgRNA sequences were designed with CRISPick ( https://portals.broadinstitute.org/gppx/crispick/public ). .. The sequences of the crRNAs and sgRNAs used in this study are described in the Appendix Table S1. pXR002: EF1a-dCasRx-2A-EGFP (Addgene plasmid # 109050; http://n2t.net/addgene:109050 ; RRID:Addgene_109050) and Article Title: Adeno-associated viral vector resource for the RNA-targeting Cas13d: A comparison of high-fidelity variants, DjCas13d and hfCas13d Article Snippet: The DNA sequence of RfxCas13d (CasRx) was derived from pXR001: EF1a-CasRx-2A-EGFP, Addgene plasmid no. 109049. .. CasRx-compatible gRNA expression cassettes used in this study were derived from Article Title: RSRC2 is a novel RNA-binding protein that safeguards mitotic fidelity by interacting with the lncRNA C1QTNF1-AS1 Article Snippet: .. Five micrograms of Article Title: Adeno-Associated Viral Vector Resource for the RNA-Targeting Cas13d: A Comparison of High-Fidelity Variants, DjCas13d and hfCas13d Article Snippet: The DNA sequence of 399 RfxCas13d (CasRx) was derived from pXR001: EF1a-CasRx-2A-EGFP, Addgene plasmid #109049. .. CasRx-compatible 400 gRNA expression cassettes used in this study were derived from Article Title: U2AF1 mutations rescue deleterious exon skipping induced by KRAS mutations Article Snippet: Band intensities were quantified using the Analyze Gels feature in Fiji (ImageJ) and used to calculate the percent spliced in for KRAS exon 2 or exon 3. .. pXR002: EF1a-dCasRx-2A-EGFP and Article Title: The RNA-binding protein RSRC2 promotes mitotic fidelity by interacting with the lncRNA C1QTNF1-AS1 Article Snippet: .. 5 μg of Article Title: RSRC2 is a novel RNA-binding protein that safeguards mitotic fidelity by interacting with the lncRNA C1QTNF1-AS1. Article Snippet: .. Five micrograms of Article Title: CRISPR/CasRx suppresses KRAS-induced brain arteriovenous malformation developed in postnatal brain endothelial cells in mice. Article Snippet: For pAAV-CAGHA-KRASG12D and pAAV-CAG-CasRx-Myc, HA-KRASG12D and CasRx-Myc amplified from pXR001:EF1a-CasRx-2A-EGFP (109049, Addgene) were subcloned into pAAV-CAG-tdTomato in a substitution of tdTomato. .. For pAAV-U6-gRNA-KRASG12D, gRNA-KRASG12D (5′-TAGTTGGAGCTGATGGCGTAGG-3′) was subcloned into Expressing:Article Title: Adeno-associated viral vector resource for the RNA-targeting Cas13d: A comparison of high-fidelity variants, DjCas13d and hfCas13d Article Snippet: The DNA sequence of RfxCas13d (CasRx) was derived from pXR001: EF1a-CasRx-2A-EGFP, Addgene plasmid no. 109049. .. CasRx-compatible gRNA expression cassettes used in this study were derived from Article Title: Adeno-Associated Viral Vector Resource for the RNA-Targeting Cas13d: A Comparison of High-Fidelity Variants, DjCas13d and hfCas13d Article Snippet: The DNA sequence of 399 RfxCas13d (CasRx) was derived from pXR001: EF1a-CasRx-2A-EGFP, Addgene plasmid #109049. .. CasRx-compatible 400 gRNA expression cassettes used in this study were derived from Derivative Assay:Article Title: Adeno-associated viral vector resource for the RNA-targeting Cas13d: A comparison of high-fidelity variants, DjCas13d and hfCas13d Article Snippet: The DNA sequence of RfxCas13d (CasRx) was derived from pXR001: EF1a-CasRx-2A-EGFP, Addgene plasmid no. 109049. .. CasRx-compatible gRNA expression cassettes used in this study were derived from Article Title: Adeno-Associated Viral Vector Resource for the RNA-Targeting Cas13d: A Comparison of High-Fidelity Variants, DjCas13d and hfCas13d Article Snippet: The DNA sequence of 399 RfxCas13d (CasRx) was derived from pXR001: EF1a-CasRx-2A-EGFP, Addgene plasmid #109049. .. CasRx-compatible 400 gRNA expression cassettes used in this study were derived from |